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Winter 1996 Newsletter


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Mycoplasma Hyopneumoniae Serology

The serology services laboratory is pleased to announce that an ELISA test for antibodies against Mycoplasmahyopneumoniaehas been developed by Dr. Kanitz at the PurdueADDL and was brought "on line" December 1st

During the last year or so, the ADDL has received many requests from swine practitioners for M.hyo.serology, to be used along with the currently available PRRSV and SIV tests as an aid in management of swine respiratory disease. This new test is in answer to these requests.

Since M.hyo.infections are nearly ubiquitous in the general swine population, it was thought that a simple positive/negative serology result would be less than adequate. Some sort of quantification would be desirable in order to assess the relative immune status of groups of pigs and to determine when and where infections are occurring. To this end the new M. hyo.ELISA test has been set up and calibrated to give numerical results similar to those of the PRRSV ELISA test. Results will be reported as S/P ratios as well as positive/negative. As with the PRRSV test, the S/P cut off point has been set at 0.40.

Although the new M. hyo. ELISA test has been developed using standard, accepted ELISA technology and high quality reagents, it has not yet been fully evaluated for sensitivity and specificity. Results accumulated to date have shown clear-cut differences between positive field samples and a large bank of known (or presumed) negative field and experimental samples.

The M. hyo. ELISA will be priced as a standard mammalian serology test; that is, $2.00 per test for Indiana swine and $4.00 for out-of-state and research submissions. Testing schedules and turn around times have not yet been worked out. They will be partially dependent on the number and frequency of serums received for testing.

- by Charles L.Kanitz, DVM, MS, PhD Chief of Virology and Serology, ADDL

Locations


ADDL-West Lafayette:
406 S. University
West Lafayette, IN 47907
Phone: 765-494-7440
Fax: 765-494-9181

ADDL-SIPAC
11367 E. Purdue Farm Road
Dubois, IN 47527
Phone: (812) 678-3401
Fax: (812) 678-3412

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