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UPDATE ON NEW TESTS FROM
 THE BACTERIOLOGY LABORATORIES AT ADDL 

New antibiotic sensitivity panels for bovine and porcine isolates

  Effective August 21, 2000 and in addition to our existing antimicrobial sensitivity break point (BK) panels (Table 1) for food animals (mainly bovine and porcine), the bacteriology laboratory will be offering a new panel (Table 2) which will provide minimum inhibitory concentrations (MIC).  The cost for sensitivity tests remains the same if only one panel is tested for each isolate.  Please indicate on your submission form if you wish to test your isolates against the MIC or BK panel.  If not indicated, the BK panel will be used.   As you will notice, the new panel has replaced tetracycline (TC) with chlortetracycline (CTC) or oxytetra-cycline (OTC).  This change is based on recent data indicating that there are poor correlations between in vitro efficacy of TC and that of  CTC or OTC.  Since TC has not been effective and there are limited spaces on the plates, TC was removed from the panel.  In addition, more sulfa drugs have been added to the new panel while amikacin, novobiocin, cephalothin and sarafloxicin were removed.  The new panel will allow  veterinarians to choose the most effective and economic antibiotics.

Table 1 (Break Point) 

         Antibiotic

Concentration range (ug/ml)

Amikacin

16,32

Florfenicol

0,5,1

Apramycin

8,16

Novobiocin

4

Penicillin

0.03,0,12,2

Cephalothin

8,16

Tilmicosin

8,16

Clindamycin

0.5,2

Enrofloxacin

0.5,1

Erythromycin

0.5,4

Gentamicin

4,8

Oxacillin+2% Nacl

2,4

Ampicillin

0.25,2,4,8

Spectinomycin

8,16

Sulphachloropyridazine

20,40

Sarafloxacin

0.06,0.12

Tetracycline

4,8

Tiamulin

8,16

Trimethroprim/Sulphadiazine

0.5/9.5,2/38

Tylosin

5,10

Ceftiofur

1,2,4

Neomycin

8

Table 2 (MIC)

         Antibiotic

Concentration range (ug/ml)

Ceftiofur

0.5,8

Erythromycin

0.25-4

Chlortetracycline

0.5-8

Florfenicol

0.25-8

Penicillin

0.12-8

Ampicillin

0.25-16

Apramycin

4-32

Sulphadimethoxine

32-256

Neomycin

4-32

Sulphachloropyridazine

32-256

Tylosin Tartrate

2.5-20

Sulphathiazole

32-256

Spectinomycin

8-64

Tilmicosin

4-32

Clindamycin

0.25-2

Tiamulin

4-32

Enrofloxacin

0.12-2

Gentamicin

1-8

Trimethroprim/Sulphamethoxazole

0.5/9.5,2/38

Oxytetracycline

0.25-8

 

Locations


ADDL-West Lafayette:
406 S. University
West Lafayette, IN 47907
Phone: 765-494-7440
Fax: 765-494-9181

ADDL-SIPAC
11367 E. Purdue Farm Road
Dubois, IN 47527
Phone: (812) 678-3401
Fax: (812) 678-3412

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