New Test: Multiplex PCR for the Detection
and salmonellaspp. from Porcine Intestinal Specimens
Proliferative enteritis, swine dysentery, and porcine
salmonellosis are the most common enteric bacterial diseases
affecting pigs in the growing and finishing stages of production.
Currently, diagnosis of these diseases by standard cultural
techniques of intestinal specimens can be laborious, time
consuming and expensive (swine dysentery, porcine salmonellosis)
or impossible (proliferative enteritis). The bacteriology
laboratory at ADDL has established a multiplex PCR assay,
using DNA sequences specific for the three intestinal pathogens,
for the diagnosis of these commonly found bacterial enteritis
in swine. The test is $15.00 per sample.
The infected segment of intestine should be tied up at
both ends, cut off, transferred to a clean whirlpak bag,
and submitted to the laboratory under refrigerated
temperature as soon as possible or no later than 24 hours.
Upon receiving the intestine, mucosal scrapings will be
collected for PCR.
The test is sensitive than standard culture techniques
for S.hyodysentariae and salmonella and
more sensitive than
histopathological examination for L. intracellularis.
Since the key element to rational therapy and effective
treatment is dependent upon rapid and accurate detection
and identification of etiologic agents in intestinal specimens,
the newly offered test will enhance the capability of field
veterinarians to better serve their clients.