General Information
NVSL Submissions
ADDL Submissions
General Serology Information
  • Please complete an appropriate Submission Form, available on the Forms page, and submit with sample.
  • All forms must be complete and legible.
  • All regulatory charts must include the submitting veterinarian’s signature.
  • All regulatory charts must include complete animal identification.
  • CF.363 Serology Test Request (Buck Slip) must accompany all regulatory charts (available from ADDL).
  • Advance notice should be given when submitting large groups of serum samples.
  • Samples delivered to the ADDL Serology Laboratory by the owner must be sealed using the veterinarian’s label or tape bearing the veterinarian’s signature.
  • Be aware that verification of any non-negative samples will cause a delay in the availability of results.
  • Samples must be received before noon on the day BEFORE the test is scheduled to be set (started). Please see pages 31–33 of the User's Guide for individual test schedules.
  • If multiple tests are requested, allow extra time for completion.
  • Testing schedules may change to accommodate caseloads and/or holidays.
  • Tests performed at the National Veterinary Services Laboratory (Ames, Iowa) must be submitted through ADDL.
  • Exports — the submitting veterinarian is responsible for informing ADDL of any special requirements (e.g., dilutions, test method), using CF.363 Serology Test Request (Buck Slip). If this information is not supplied, the standard test will be performed.
  • Test regulations — for information please call: Federal-USDA Area Veterinarian in Charge Office of the State Veterinarian (317)347-3100 (317)227-0300.
  • If there are any questions concerning submissions, please contact the ADDL Serology office at (765)494-7451 prior to submitting samples.
Serum submitted to Purdue ADDL for the following tests will be forwarded to the National Veterinary Services Laboratory in Ames, Iowa.
Brucella melitensis
3% card
Brucella ovis
Malignant Catarrhal Fever
Q fever
Equine Influenza (Prague, KY, Miami)
Equine Encephalomyelitis (EEE,WEE,VEE)
VN EEE also by IgM ELISA
West Nile Virus (WNV)
Submitting Serum Samples to ADDL
  • Please complete a CF.369 Regular Submission Form, CF.392 Neoplasm Submission Form or CF.363 Serology Test Request (Buck Slip) and submit with sample. Forms are available on the Forms page.
  • Submitting clear serum in 5 ml cap tube (size 12x75) is preferred.
  • Each tube must be identified by at least a tube number and contain at least 1 ml of clear serum; further identification is preferred.
  • Tube numbers and numbers on chart must match and be in consecutive order (no skipped numbers).
  • Acute and convalescent samples of serum should be submitted together. ADDL will not hold single samples pending a later submission.
Acceptable Forms for Submitting Serum Samples to ADDL
  • Form VS4-33 Brucellosis Test Record
    Usage: All regulatory testing
    Supplied by: State Board of Animal Health (317-227-0300)
  • Certificate of Veterinary Inspection (Health Certificate)
    Usage: All show, interstate movement and sale testing
    Supplied by: State Board of Animal Health (317-227-0300)
  • Form VS10-11 Equine Infectious Anemia Laboratory Test Record
    Usage: EIA testing only
    Supplied by: State Board of Animal Health (317-227-0300)
  • CF.369 Regular Submission Form
    Usage: All diagnostic testing Pages 1 and 3 of this form must be completed
    Supplied by: Animal Disease Diagnostic Laboratory
    Downloadable version: Accession Form
  • CF.363 Serology Test Request (Buck Slip)
    Usage: Must be completed and attached to all regulatory test records and health certificates (ADDL will run ONLY those tests requested on this form. It is not necessary to attach a buckslip to CF.369 Regular Submission Form [diagnostic testing], or EIA test charts
    Supplied by: Animal Disease Diagnostic Laboratory
Test Abbreviations
Agar gel immunodiffusion
Buffered acidified plate antigen
Enzyme Linked Immunosorbent Assay
Competitive Enzyme-Linked Immunosorbent Assay
Hemagglutination inhibition
Indirect fluorescent antibody assay
Slide agglutination
Standard plate test
Standard tube test
Virus neutralization