Quick Reference
Sample Submissions
The following are suggestions for etiologic agents to consider when determining diagnostic procedures to request related to specific disease syndromes. While not comprehensive, it will offer guidelines for choosing appropriate diagnostic samples to submit for specific tests.
It is advisable to submit freshly dead or symptomatic, untreated whole animal carcasses for a complete post mortem examination. In addition to the chilled fresh samples listed, it is usually advisable to submit formalin-fixed tissues for histopathologic examination. For questions concerning any diagnostic procedure, call the Purdue ADDL located on Purdue’s campus in West Lafayette, IN at (765)494-7440 or the Heeke ADDL located in Dubois, IN at (812)678-3401.
Key
AGID
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Agar gel immunodiffusion
CF
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Complement fixation
ELISA
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Enzyme Linked Immunosorbent Assay
EM
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Electron microscopy
FA
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Fluorescent antibody
IFA
-
Indirect fluorescent antibody
PCR
-
Polymerase chain reaction
VI
-
Virus isolation
VN
-
Virus neutralization
Respiratory References
AgentSpecimenProcedure
BRSV (Bovine Respiratory Syncytial   virus)Affected lung portions, tracheaFA
BRSV (Bovine Respiratory Syncytial   virus)Serum (acute and convalescent)Serology
BVDV (Bovine Viral Diarrhea virus)Buffy coat (EDTA blood)VI, PCR
BVDV (Bovine Viral Diarrhea virus)Lung, intestine, spleen, fetal organsFA, VI, PCR
BVDV (Bovine Viral Diarrhea virus)Serum (Acute and convalescent)Serology, PCR
IBR (Infectious Bovine Rhinotracheitis virus)Nasal secretions, trachea, lung, spleenFA, VI
IBR (Infectious Bovine Rhinotracheitis virus)Fetal lung, spleen, kidney, liverFA, VI
IBR (Infectious Bovine Rhinotracheitis virus)Serum (acute and convalescent)Serology
PI3  (Parainfluenza virus)Nasal secretions, trachea, lung, spleenFA, VI
PI3  (Parainfluenza virus)Fetal lung, spleen, kidney, liverFA, VI
PI3  (Parainfluenza virus)Serum (acute and convalescent)Serology