|Necropsy||Maximum number of animals (similar age and clinical problem) to be included in 1 accession:|
Cattle: 1 adult or 2 calves
Cats: 1 adult or 3 kittens
Dogs: 1 adult or 3 puppies
Horses: 1 adult or 2 foals
Sheep: 1 adult or 3 lambs
Swine: 1 adult or 3 pigs
Abortion/Stillbirth: 3 fetuses w/placenta
|Shipping containers for mail or courier services must be leak-proof and airtight||-All species
-Carcasses must be held at refrigeration temperature until delivery to ADDL.
-DO NOT freeze unless interval between death and delivery > 3 days.
-Carcasses smaller than 150lbs can be delivered after hours to the walk-in refrigerator on the south dock (be sure to place red hold tag with owner's name, date and contact information on animal before placing in white cooler).
-After hours, if delivering an animal over 150lbs and will not fit in the white cooler please contact ADDL at 765-494-7440 to be put in contact with the pathologist on duty.
-Gross necropsy results will be reported in 1-5 working days; final reports are completed within 5-14 working days.
-ADDL does not euthanize companion animals, including horses.
|Cosmetic necropsy||1 animal||-Prior arrangements with ADDL are required. However, cosmetic necropsy is discouraged because it prevents thorough postmortem examination and may hinder diagnosis.
-Animal must be in ADDL before 1 pm and will usually be returned to owner the same day. Additional charge applies.
|Legal/Insurance Necropsy||1 animal||Must be designated as such prior to necropsy. Additional charge applies.
|Field Consultation||Call to schedule. Field consultations are made only at the request of a veterinarian.
|Practitioner-performed necropsy||-Brain: whole or half |
-Spinal cord: entire, with opened dura
-Endocrine glands: small, whole; larger glands, bisected
-Heart, lung, liver, kidney, spleen: 5 mm thick slices (whole heart if cardiac defect is suspected)
-GI tract: 3-5 cm segments
|10% formalin||-Brain, heart, lung, liver, kidney and spleen plus any other tissues with gross lesions should be submitted from all necropsy examinations. Other tissues should be included according to history or clinical signs.
-Formalin-fixed specimens accompanied by fresh (unfixed) specimens for ancillary testing (microbiology, toxicology, virology etc) are processed as long-form accessions. Histologic findings are reported in 3-5 working days with final reports in 7-15 days.
|Amputations||-Small specimens, e.g. digits, may be submitted whole |
-Larger specimens, e.g. limbs, should be refrigerated and delivered to ADDL within 24 hours
|10% formalin -Refrigeration for large specimens||Bony specimens will require 1-10 day delay for decalcification. Additional charges apply.
-Limb amputations can be submitted as a "Biopsy Plus" for dissection and gross examination by a pathologist before histopathology. These will be charged as a necropsy.
|Histopathology||Various tissue specimens||Leak-proof wide-mouth jar or Whirl Pak||-Tissues should be fixed in 10% buffered formalin with 10:1 formalin to tissue ratio.
-Excess formalin can be decanted after 1-2 days fixation.
|Endoscopic, pinch, core (Tru-cut), or punch biopsies||Entire biopsy specimen: if <3mm in any dimension, submit in histologic cassette to avoid specimen damage or loss||10% formalin||Multiple specimens should be submitted in individually labeled containers to indicate tissue site. Histologic findings from Neoplasm Submission Form accessions are reported in 1-3 working days. Histochemistry or immunohistochemistry requires another 1-5 working days.
|Excisional biopsies||If >2cm in diameter, slice accordingly to allow fixation||10% formalin||Use paint or suture tags to orient specimen or identify margins, if pertinent. Histologic findings from Neoplasm Submission Form accessions are reported in 1-3 working days.