Quick Reference
Sample Submissions
The following are suggestions for etiologic agents to consider when determining diagnostic procedures to request related to specific disease syndromes. While not comprehensive, it will offer guidelines for choosing appropriate diagnostic samples to submit for specific tests.
It is advisable to submit freshly dead or symptomatic, untreated whole animal carcasses for a complete post mortem examination. In addition to the chilled fresh samples listed, it is usually advisable to submit formalin-fixed tissues for histopathologic examination. For questions concerning any diagnostic procedure, call the Purdue ADDL located on Purdue’s campus in West Lafayette, IN at (765)494-7440 or the Heeke ADDL located in Dubois, IN at (812)678-3401.
Key
AGID
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Agar gel immunodiffusion
CF
-
Complement fixation
ELISA
-
Enzyme Linked Immunosorbent Assay
EM
-
Electron microscopy
FA
-
Fluorescent antibody
IFA
-
Indirect fluorescent antibody
PCR
-
Polymerase chain reaction
VI
-
Virus isolation
VN
-
Virus neutralization
Diarrhea References
AgentSpecimenProcedure
BVD (Bovine viral diarrhea)Spleen, mesenteric lymph node, rumen, ileumFA, VI
BVD (Bovine viral diarrhea)Buffy coat (Submit EDTA blood for acutely infected animals and persistently infected calvesVI, AgcELISA
Serology
BVD (Bovine viral diarrhea)SerumPCR
BVD (Bovine viral diarrhea)Lymph node, spleen, blood, serum 
CoronavirusSpiral colon, ileum, fecesFA +/-
VI
CoronavirusFecesEM
CoronavirusFeces, intestinal contentsRT-PCR
RotavirusUpper, middle, lower small intestine, 6” segments tied offFA, VI
EM, AgcELISA
Rotavirus  (DO NOT OPEN GUT) 
RotavirusFeces