Bacteriology
General Information
The Microbiology Service of the ADDL provides examinations for a wide variety of diseases affecting the animal population of Indiana. Some of the most common are listed in the Fees pages. In addition to those services listed, the following are available:
  • Please complete an appropriate Submission Form, available on the Forms page, and submit with sample.
  • Antibiotic susceptibility tests of recovered isolates - Please indicate on the accession form that you are requesting susceptibility testing.
  • Identification of bacterial isolates - PLEASE SEND ONLY PURE CULTURES FOR IDENTIFICATION. If mixed cultures are received, only the most predominant colony type will be identified unless sender specifies otherwise.
  • Serogrouping of Salmonella - Salmonella isolates will be checked for serogroups at the ADDL and the isolate forwarded to NVSL for definitive typing. A final report from NVSL may take four weeks or longer.
  • Return or forwarding of isolates recovered from clinical material - If you would like this service, the request to save the isolate must be made at the time of submission. Be specific as to which isolate you would like saved. ADDL will forward isolates to reference laboratories (Submitter will be asked to complete paperwork for the reference laboratory, and fax it to ADDL prior to shipment of isolate; postage charges will be incurred).
  • All Streptococcus suis isolates are checked against antisera 1-34 unless requested otherwise.
  • Actinobacillus pleuropneumoniae isolates are checked against antisera 1-12.
  • Some tests that are not performed at Purdue ADDL are available at other laboratories.
    • Cultural examination for Trichomonas is done at the University of Wisconsin. Purdue will accept the commercial "one pouch" system and provide interpretation.
    • Samples suspected of Contagious Equine Metritis (CEM) will be forwarded to NVSL for processing. The Breathitt Veterinary Center, Hopkinsville, Kentucky, will accept submissions for cultural examination for CEM. Specimens must be submitted in Aimes Transport Medium. Call the Breathitt Center at 270-886-3959 prior to submitting samples.
  • Specimens suspected of harboring anaerobic bacteria must be shipped in an anaerobic container.
Collection and Care of Milk Samples
Valid Bacteriology laboratory results for mastitis control require that contaminating bacteria be kept to an absolute minimum. This requirement is relatively difficult to achieve since 1) the cow's environment is rich in bacterial contaminants and 2) milk is an excellent culture medium. Bacterial contaminants can be kept to a minimum by following these precautions.
  • Collect the milk aseptically.
    • Brush dirt off the cow's flanks.
    • Scrub the udder with a surgical detergent, rinse well, and dry with clean paper towels. The order of washing, rinsing and drying should be the far side first and the near side last.
    • Apply iodine or alcohol to the teat orifice and allow to dry.
    • Discard three or four streams of milk into CMT paddle and test. Collect 5-10mls from quarters with 2+ CMT value (or greater) into a sterilized screw-capped tube tiled to minimize contamination by airborne bacteria. Collect specimens from near quarters first and far quarters last.
  • Tighten the screw cap, label with an indelible marker (cow number and quarter), and immediately place in cooler.
  • Keep the specimens cold until they are delivered to the laboratory. Delivery should be made on the day of collection.
  • If you are planning to submit more than eight milk specimens at one time, please notify the ADDL to schedule the submission so that adequate time is allowed for preparation of the necessary culture media.
  • If samples were frozen, please indicate on the submission form. A larger sample size is necessary for culture to compensate for the loss of a log of organism through freezing and thawing.