Quick Reference
Sample Submissions
The following are suggestions for etiologic agents to consider when determining diagnostic procedures to request related to specific disease syndromes. While not comprehensive, it will offer guidelines for choosing appropriate diagnostic samples to submit for specific tests.
It is advisable to submit freshly dead or symptomatic, untreated whole animal carcasses for a complete post mortem examination. In addition to the chilled fresh samples listed, it is usually advisable to submit formalin-fixed tissues for histopathologic examination. For questions concerning any diagnostic procedure, call the Purdue ADDL located on Purdue’s campus in West Lafayette, IN at (765)494-7440 or the Heeke ADDL located in Dubois, IN at (812)678-3401.
Key
AGID
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Agar gel immunodiffusion
CF
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Complement fixation
ELISA
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Enzyme Linked Immunosorbent Assay
EM
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Electron microscopy
FA
-
Fluorescent antibody
IFA
-
Indirect fluorescent antibody
PCR
-
Polymerase chain reaction
VI
-
Virus isolation
VN
-
Virus neutralization
Abortion References
AgentSpecimenProcedure
Bluetongue virus (BT)SerumSerology
BVDV (Bovine viral diarrhea virus)Fetal thoracic/abdominal fluidSerology (VN)
BVDV (Bovine viral diarrhea virus)Placenta, fetal lung, spleen, kidney, brain, intestine, lymph node, serumFA, VI, PCR, IHC
BVDV (Bovine viral diarrhea virus)Serum from dam (acute and convalescent)Serology
IBR (Infectious Bovine Rhinotracheitis virus)Fetal thoracic/abdominal fluidSerology (VN)
IBR (Infectious Bovine Rhinotracheitis virus)Placenta, fetal lung, spleen, kidney, brain, intestine, lymph nodeFA, VI
IBR (Infectious Bovine Rhinotracheitis virus)Serum from dam (acute and convalescent)Serology
Neospora Serum (from dam)Serology
Neospora Any tissue with lesionsPCR
PI3 (Parainfluenza virus)Fetal thoracic/abdominal fluidSerology (VN)
PI3 (Parainfluenza virus)Placenta, fetal lung, spleen, kidney, brain, intestine, lymph nodeFA, VI
PI3 (Parainfluenza virus)Serum from dam (acute and convalescent)Serology