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PCR (Polymerase-chain-reaction) Tests
The following is a list of PCR Tests currently available.
Organism
Test Type
Suggested Samples
Influenza viral typing: H1N1, H1N2, H3N2
RT-PCR, 2 tests
Viral isolates only; first requires virus isolation on lung
BVD1 and BVD2 viruses
RT-PCR, nested
Lymph nodes, spleen
PRRS virus
RT-PCR, nested or Real Time PCR
Serum, spleen, lung
TGE virus (differentiates from PRCV)
RT-PCR, nested or Real Time PCR
Small intestine, feces
Leptospira sp. (all pathogenic species)
PCR
Urine, liver, kidney
Chlamydia sp. (genus specific)
PCR
Placenta, liver
Chlamydia psittaci
PCR
Placenta, liver, other
Neosporacaninum
PCR
Any tissue with lesions
West Nile virus (mammals)
RT-PCR, nested
Brain and spinal cord
West Nile Virus (avian)
PCR
Kidney, heart, brain, liver, spleen
Infectious Bursal Disease virus
PCR
Bursa
Turkey Coronavirus
PCR
Small intestine, feces
Mycoplasmagallisepticum
PCR
Lung, trachea, oviduct, swabs from sinuses, choana, and trachea
Mycoplasmasynovium
PCR
Lung, trachea, oviduct, swabs from sinuses, choana, trachea and joints
Lawsonia intracellularis(1)
PCR
Ileal mucosa, feces
Brachyspira hyodysenteriae(2)
PCR
Cecal and colonic mucosa, feces
Brachyspira pilosicoli(3)
PCR
Cecal and colonic mucosa, feces
Salmonella sp.(4) (genus specific)
PCR
Intestinal mucosa, feces, other tissues as indicated
Enteric panel (1-4 above)
PCR (multiple)
Intestinal mucosa, feces
E. coli virulence typing panel:F18, 987P and F41 pili; eae attachment gene; LT, StaP, Stb, an
PCR (multiple)
Bacterial isolates only; first requires bacterial culture
Clostridium perfringens typing panel: a, b, e, i and b2 toxins;  enterotoxin
PCR (multiple)
Bacterial isolates only; first requires bacterial culture
Clostridium difficile toxins A and B
PCR
Frozen colonic contents from animals with minimal autolysis
Mycobacterium paratuberculosis
PCR
Mucosa from ileum, colon, ileocolic valve, mesenteric lymph nodes
Listeriamonocytogenes
PCR
Medulla oblongata
Renibacteriumsalmonarium
PCR
Kidney
Myxoboluscerebralis
PCR
Hemi-section of the head
Porcine Circo-Virus
PCR
Lung, Spleen, Lymph Node, Serum
N. risticii
Nested PCR
Lymph Node, Spleen, Interstinal Contents

 

Additional Links for this Section:

What is PCR
Submitting Samples
Recommended Uses and Interpretation

Locations


ADDL-West Lafayette:
406 S. University
West Lafayette, IN 47907
Phone: 765-494-7440
Fax: 765-494-9181

ADDL-SIPAC
11367 E. Purdue Farm Road
Dubois, IN 47527
Phone: (812) 678-3401
Fax: (812) 678-3412

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