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PCR Testing by Request Only
PCR tests are run by request only. Additional charges will
be incurred for each PCR test that is run on samples that
are mailed to ADDL and on tissues collected during necropsy
examination at ADDL (see below). When PCR tests are desired
for various agents, each desired test must be clearly requested
on the ADDL Submission Form at the bottom of the first page
under either “Clinical Diagnosis” or “Other
specified tests”. Alternatively for only animals submitted
for necropsy, permission may be granted in writing as follows,
“Please run any PCR tests that are deemed necessary
for diagnosis”.
Large Numbers of Samples by Prior Arrangement Only
Prior arrangements are required for submissions of > 10
samples from the same source on the same day for PCR testing.
Please contact us at the general ADDL telephone number (765)
494-7440 and you will be forwarded to the appropriate laboratory
Special Care Needed in Collection of Samples for PCR Testing
PCR tests provide unprecedented sensitivity and specificity.
However, due to the extreme sensitivity of PCR, cross-contamination
of samples is common. It is best to collect samples with disposable
instruments (plastic tableware often works well) into sterile
whirl-paks. Optimum sample size per test is approximately
10-15 grams or mls (1/2-1.0 cubic inch). Eliminate air, roll
the tops down and seal by folding tabs. Avoid use of instruments
that may be contaminated from previous use. (Whirl-pak bags
are available for purchase at ADDL [$5.00/50] or through various
scientific suppliers such as VWR Scientific Products [1-800-932-5000],
PGC Scientifics [1-800-424-3300], Fisher Scientific [1-800-766-7000]).
Special Packaging Required for PCR Testing
Tissues sent for PCR must be packaged separately in whirl-paks
and clearly labeled with which tissues are included and tests
requested e.g. “Liver, Kidney” “for Lepto
PCR”. Samples should be shipped overnight on ice-packs
in an insulated shipping container. All tissue samples in
a single bag will be pooled and a single PCR test will be
run for each requested agent. If the same PCR test is desired
on tissues from different animals or from different tissues
from the same animal, then separate samples in separate whirl-paks
must be submitted. |
Abortions
