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Summer 2000 Newsletter


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Johne’s PCR

  While we have passed the check test for Johne’s culture by the National Veterinary Services Laboratory (NVSL) for the past three years with high marks, we still want to improve the turnaround time for diagnosing

Johne’s disease.  A PCR test has been developed and validated.  It will be applied to the bacterial culture tubes for fecal specimens at two to six weeks after incubation.  Please note that we do not recommend eliminating the culture but, if you need a faster presumptive diagnosis, the PCR can help identify the positive shedders more quickly.  Based on our experience, we can detect bacterial colonies at the 8 week culture tubes; however, we can detect positive results for Johne’s disease by PCR from the same tube at 2-3 weeks after incubation before any colonies become visible.  For low shedders, it generally takes 12-16 weeks for the bacterial colonies to be visible in the culture tubes; a swab taken from the same tube at 4-6 weeks after incubation would give positive PCR results for Johne’s.  If you need a quicker presumptive diagnosis, it would be beneficial to request PCR in addition to the routine culture request.  There will be an additional charge of $15.00 for each PCR requested.

Information needed

  We would like to take this opportunity to thank our users for their efforts in completing submission forms in detail and providing us with differentials whenever possible.  This enables us to use the most appropriate reagents/media for bacterial isolation and identification.  Please continue to provide with as much history, treatment and other relevant information as possible.

-by Ching Ching Wu, Head of Bacteriology

Locations


ADDL-West Lafayette:
406 S. University
West Lafayette, IN 47907
Phone: 765-494-7440
Fax: 765-494-9181

ADDL-SIPAC
11367 E. Purdue Farm Road
Dubois, IN 47527
Phone: (812) 678-3401
Fax: (812) 678-3412

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