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General Pathology Information

Procedure Specimen Container Comments
Necropsy Maximum number of animals (similar age and clinical problem) to be included in 1 accession:
Cattle:   1 adult or 2 calves
Cats:     1 adult or 3 kittens
Dogs     1 adult or 3 puppies
Horses: 1 adult or 2 foals
Sheep:  1 adult or 3 lambs
Swine:   1 adult or 3 pigs Abortion/Stillbirth:  3 fetuses w/placenta
Shipping containers for mail or courier services must be leak-proof and airtight  -All species -Carcasses must be held at refrigeration     temperature until delivery to ADDL. -DO NOT freeze unless interval between death and delivery > 3 days. -Carcasses smaller than 150 lb. can be      delivered after hours to the walk-in refrigerator on the south dock (be sure to identify with name, contact phone number). -Gross necropsy results will be reported in 1-5 working days; final reports are completed within 5-14 working days. -ADDL does not euthanize companion animals, including horses.
Cosmetic necropsy 1 animal   -Prior arrangements with ADDL are required.  However, cosmetic necropsy is discouraged because it prevents thorough postmortem  examination and may hinder diagnosis. -Animal must be in ADDL before 1 pm and will usually be returned to owner the same day.  Additional charge applies.
Legal/Insurance Necropsy 1 animal   Must be designated as such prior to necropsy.  Additional charge applies.
Field Consultation     Call to schedule.  Field consultations are made only at the request of a veterinarian.
Practitioner-performed necropsy -Brain: whole or half -Spinal cord: entire, with opened dura -Eyes: whole -Endocrine glands: small, whole; larger glands, bisected -Heart, lung, liver, kidney, spleen: 5 mm thick slices (whole heart if cardiac defect is         suspected) -GI tract: 3-5 cm segments 10% formalin -Brain, heart, lung, liver, kidney and spleen plus any other tissues with gross lesions should be submitted from all necropsy examinations.  Other tissues should be included according to history or clinical signs. -Formalin-fixed specimens accompanied by fresh (unfixed) specimens for ancillary testing (microbiology, toxicology, virology etc) are  processed as long-form accessions.  Histologic findings are reported in 3-5 working days with final reports in 7-15 days.
Amputations -Small specimens, e.g. digits, may be submitted whole -Larger specimens, e.g. limbs, should be refrigerated and delivered to ADDL within 24 hours 10% formalin -Refrigeration for large specimens Bony specimens will require 1-10 day delay for decalcification.  Additional charges apply. -Limb amputations can be submitted as a "Biopsy Plus" for dissection and gross         examination by a pathologist before           histopathology.  These will be charged as a necropsy.
Histopathology Various tissue specimens Leak-proof wide-mouth jar or Whirl Pak -Tissues should be fixed in 10% buffered     formalin with 10:1 formalin to tissue ratio. -Excess formalin can be decanted after 1-2 days fixation.
Endoscopic, pinch, core (Tru-cut), or punch    biopsies Entire biopsy specimen: if <3mm in any      dimension, submit in histologic cassette to avoid specimen damage or loss 10% formalin Multiple specimens should be submitted in individually labeled containers to indicate tissue site. Histologic findings from Neoplasm Submission Form accessions are reported in 1-3 working days.  Histochemistry or immunohistochemistry     requires another 1-5 working days.
Excisional biopsies If >2cm in diameter, slice accordingly to allow fixation 10% formalin Use paint or suture tags to orient specimen or identify margins, if pertinent.  Histologic findings from Neoplasm Submission Form accessions are reported in 1-3 working days.

 

Locations


ADDL-West Lafayette:
406 S. University
West Lafayette, IN 47907
Phone: 765-494-7440
Fax: 765-494-9181

ADDL-SIPAC
11367 E. Purdue Farm Road
Dubois, IN 47527
Phone: (812) 678-3401
Fax: (812) 678-3412

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