Topics of Interest
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7/30/2014 - PEDV Pooling now available at the ADDL
PEDV Pooling now available at the ADDL
After analyzing the data from our recent PEDV Pooling Study, pooling of up to 5 fecal samples from a clinically affected herd for PEDV PCR has been approved in the ADDL.
When submitting samples for PEDV, please indicate on your submission form if you wish to have fecal samples pooled or tested individually. All samples will be tested individually unless pooling is requested.
Samples from pools testing Positive or Suspect must be tested individually to identify Positive or Suspect animals.
The cost for PEDV PCR is $30.00 plus a $10.00 accession fee.
Swabs will be tested individually only, as pooling may result in false negative results.
Pooling is not available for PDCoV at this time, but studies are underway to determine if pooling is an acceptable option.
Please call the ADDL at 765-494-7440 if you have any questions.
7/22/2014 - Porcine Delta Coronavirus PCR Now Offered at ADDL
Porcine Delta Coronavirus PCR Now Offered at ADDL
The ADDL can now diagnose the presence of Porcine Delta Coronavirus (PDCoV) by PCR in any age pigs or environmental samples.
Samples to submit are:
  • Jejunum and ileum, 6-8” segments, fresh (for PCR) and formalin-fixed
  • Spiral colon, fresh (for PCR – package separately from jejunum/ileum)
Please call the ADDL at 765-494-7440 if you have any questions.
For more information on Swine Enteric Coronavirus Diseases (SECD), including Porcine Epidemic Diarrhea virus (PEDv) and Porcine Delta Coronavirus (PDCoV) please see the USDA SECD site: http://www.aphis.usda.gov/wps/portal/aphis/ourfocus/animalhealth and click on “Porcine Epidemic Diarrhea Virus”.
7/18/2014 - Turkey Coronavirus PCR Now Offered at ADDL
Turkey Coronavirus PCR Now Offered at ADDL
The ADDL can now diagnose the presence of Turkey Coronavirus (TCV) by PCR.
Samples to submit from affected turkeys are: cloacal swabs, intestinal tissues, or feces.
The cost for this test is $30.00 plus $10.00 accession fee.
Please call the ADDL at 765-494-7440 if you have any questions.
3/24/2014 - PEDv Validation Study UPDATE
PEDv Validation Study UPDATE
We are currently looking for individuals to participate in a PEDv validation study that will determine if PEDv can be detected in pooled samples of 3 or 5. If you are interested in additional information and or participating, please contact Aimee Highland at ahighlan@purdue.edu or 765-494-7448.
UPDATE: For more information on participating in the validation study, including instructions, please read the ValidationLetter.pdf. (If you have problems viewing the pdf, please right click and Save Target As to download a copy)
3/24/2014 - PEDv Validation Study letter
PEDv Validation Study letter
Please note, in the recent mailing, there was an incorrect date listed. The study will begin on Monday March 24, 2014.
2/18/2014 - Dr. Grant Burcham to Start at Heeke ADDL in June
Dr. Grant Burcham to Start at Heeke ADDL in June
Dr. Burcham will begin his work as the head of the Heeke ADDL lab in Dubois County on June 1st, 2014. “I am looking forward to working with the producers and animal owners in southern Indiana to help address animal health needs there. I am also excited about working alongside the excellent staff currently at the Heeke lab, “ Burcham said.
Dr. Burcham earned a Master of Science in Veterinary Pathology in 2010 and a Doctorate of Veterinary Medicine in 2007 from Purdue, and a Bachelor’s degree in Biology in 2003 from Hanover College. He is a diplomate of the American College of Veterinary Pathologists.
Dr. Burcham succeeds Dr. Duane Murphy, who retired from Purdue after nearly 20 years of service to the university.
Please join us in welcoming Dr. Burcham to the Indiana ADDL team!
1/23/2014 - PEDv PCR Now Offered at ADDL
PEDv PCR Now Offered at ADDL
The ADDL can now confirm the presence of PEDv by PCR in cases of epidemic diarrhea in any age pigs. TGE can be ruled out through PCR, or FA and rotavirus by FA or EM.
Porcine Epidemic Diarrhea Virus (PEDv)
Porcine epidemic diarrhea virus (PEDv) has been identified in commercial swine operations in the Midwest. PEDv is a coronavirus genetically and antigenically distinct from the coronavirus of transmissible gastroenteritis (TGE), which is common in parts of Asia and Europe, but is new to the U.S. PEDv causes TGE-like gastroenteric disease in all age groups of pigs, with neonates and suckling pigs being most severely affected. Samples to submit from acutely affected pigs (within the first 24 hours of onset of diarrhea) are:
  • Jejunum and ileum, 6-8” segments, fresh (for PCR) and formalin-fixed
  • Spiral colon, fresh (for PCR – package separately from jejunum/ileum)
Other samples amenable to testing for PEDv include oral fluids, rectal swabs and feces/colonic contents (fresh chilled for PCR).
Please call the ADDL at 765-494-7440 if you have any questions. Additional information can be found in the PED Talking Points (.pdf) from the USDA.
11/6/2013 - Johne's Diagnostic Update
Johne’s Diagnostics Update
Johne’s disease, a chronic intestinal disease of ruminants caused by Mycobacterium avium subsp. paratuberculosis (MAP), can be difficult to diagnose due to a delay of years between exposure and the development of clinical signs. Delayed antibody production and intermittent shedding in feces compound diagnostic difficulties. ADDL is now recommending PCR over culture as a means to detect MAP shedding in feces based on recent studies* and the performance of our laboratory and other MAP testing laboratories on the National Veterinary Service Laboratory’s (NVSL) Johne’s Disease Proficiency Test which have demonstrated that PCR is at least as sensitive as culture. In the past, culture was considered the gold standard due to inhibitors in fecal samples that interfered with the PCR tests, but new extraction methods have overcome the problem of PCR inhibitors. The benefit to our clients is the quick turn-around time of 48 hours for PCR as compared to the 7 weeks that it takes for culture. Culture will no longer be less expensive, as we have raised the price of Johne’s culture to $35 per sample to meet the rising costs of test materials and procedures, which, unbeknownst to our clients, also used PCR to confirm any suspect cultures. Keep in mind that the same interpretive problems associated with negative cultures also apply to negative PCR tests. A culture or PCR test on feces may be negative because the animal is not infected, or because the animal is infected but was not shedding the organism in feces at the time of sample collection. The difficulties of interpreting negative fecal samples for sheep and goats is amplified by the fact that small ruminants tend to shed less organism than cattle, and ADDL has an ongoing study to improve and evaluate methods for detecting fecal shedding of MAP in small ruminants. Johne’s culture is still available by request, and serologic testing remains the preferred method for herd screening. Johne’s PCR will be run in the ADDL on Wednesdays and Fridays of every week except official holidays at Purdue University to accommodate everyone. Cost for each PCR test is $35 per sample or $40 for pooled samples.
*Alinovi. “Real-time PCR, compared to liquid and solid culture media and ELISA for the detection of Mycobacerium avium ssp. paratuberculosis.” Veterinary Microbiology. 2009

*Collins. “Diagnosis of paratuberculosis.” Veterinary Clinics of North America: Food Animal Practice. 2011.

*Logar. “Evaluation of combined high-efficiency DNA extraction and real-time PCR for detection of Mycobacterium avium subsp. parartuberculosis in subclinically infected dairy cattle: comparison with faecal culture, milk real-time PCR and milk ELISA.” BMC Veterinary Research. 2012.
8/26/2013 - ADDL at the Purdue Vet Med Fall Conference September 11-13, 2013
Animal Disease Diagnostic Laboratory (ADDL) 100 Year Anniversary
Come celebrate as we mark our 100th year of animal disease testing in Indiana! Visit our booth (#39) and reminisce with us as we look back over the last 100 years. Grab a bag to carry your conference materials in as our thank you for your support. Help us help you by completing a brief survey on the Animal Disease Diagnostic Laboratory and be entered for a chance to win an Apple iPad mini! Want a closer look? Tours of the ADDL lab will be offered! Stop by and see us (2nd Floor Stewart Center) to get scheduled for a tour.
For more information visit the PVM 2013 Fall Conference.
8/6/2013 - New Field Necropsy Test Available
New Field Necropsy Test Available
Field Necropsy $115.00
Includes Histopathology (up to 8 slides), two routine Bacteriology tests and one antimicrobial susceptibility and routine Virology. Does not include Toxicology or Molecular Diagnostic tests. If samples from multiple animals are submitted, specimens will be pooled for Bacteriology and Virology testing.
5/20/2013 - Porcine Epidemic Diarrhea Virus (PEDV)
Porcine Epidemic Diarrhea Virus (PEDV)
Porcine epidemic diarrhea virus (PEDV) was recently identified in four commercial swine operations in the Midwest. PEDV is a coronavirus genetically and antigenically distinct from the coronavirus of transmissible gastroenteritis (TGE), which is common in parts of Asia and Europe, but is new to the U.S. PEDV causes TGE-like gastroenteric disease in all age groups of pigs, with neonates and suckling pigs being most severely affected.
The ADDL can perform histopathology, TGE PCR, TGE FA, rotavirus FA, and EM to look for, and rule out TGE and rotavirus. At this time, TGE and rotavirus negative samples can then be sent to NVSL.
Samples to submit from pigs within the first 24hrs of diarrhea are: Small intestine, spiral colon (multiple sections of each, fresh and formalin-fixed) and Feces/colonic contents (fresh-chilled and formalin-fixed).
Please call the ADDL if you have questions, and for more information, read the PED Talking Points (.pdf) from the USDA.
4/17/2013 - PRRS Same Day Results Available
PRRS Same Day Results Available
In an effort to continue to provide the best service possible, the Indiana Animal Disease Diagnostic Laboratory (ADDL) is able to offer same day results on PRRS samples if they are submitted according to the following guidelines:
  1. For same day service, communicate with ADDL staff before sending samples to the lab.
    • This will ensure that “all systems are go” for rapid turn-around time.
  2. Samples must arrive at the ADDL no later than 10:00 AM either by hand delivery or by early delivery through FedEx or UPS.
    • This is the latest that samples can arrive and allow enough time to run the test.
    • Regular next day air does not guarantee this time and samples may not be received by the 10:00 AM cut off.
  3. Indicate, “Same Day” service on the ADDL submission form and on the outside of the package.
    • Labels are enclosed for you to use.
    • It is especially important to label the outside of the package!
If arrangements are not made in advance and samples do not arrive prior to 10:00 AM, the ADDL will do its best, but cannot guarantee same day services.

Please feel free to contact us should you have questions and or concerns.
2/1/2013 - BOAH Lifts Piroplasmosis Testing Requirement
BOAH Lifts Piroplasmosis Testing Requirement
The Indiana Board of Animal Health (BOAH) has lifted the testing requirement for equine piroplasmosis (EP) at Indiana race tracks, as of Feb. 1, 2013.

Since BOAH has lifted the requirement and mandate for testing, Piroplasmosis testing at the ADDL will no longer be free. Starting on Feb 1, 2013, the fee for all testing for Piroplasmosis will be $7.00 ($7.00 for B. caballi and $7.00 for T. equi) in-state, $14.00 each out-of-state.

Please follow this link for the BOAH announcement, LSA #12-621: Repeal of Equine Piroplasmosis Testing Requirement.
1/18/2013 - New Johne's Method and Pricing
New Johne's Method and Pricing
The Mycobacterium Paratuberculosis (Johne’s) test method has changed. Effective February 1, 2013 there is a new real-time polymerase-chain-reaction (PCR) test for Johne’s which will replace the old PCR test. The cost of this test will be $35.00 for a single sample and $40.00 for pooled samples.
Should you have any questions regarding this new method, please contact our laboratory at 765-494-7440. We thank you for the opportunity to provide these services to you and look forward to serving you in the future.
8/17/2012 - Videos - ADDL in the News
Videos - ADDL in the News
The following videos, courtesy of WLFI, discuss recent drought and blue-green algae problems. For more information on these topics, please click here
The following video discusses a BSE scare in California
7/24/2012 - Drought & Toxic Effects on Animals
The Drought and Potential Poisonings in Animals in Indiana
Indiana Animal Disease Diagnostic Laboratory at Purdue University
Poisonous Plants - The Great Drought and Heat Wave of 2012 are severely damaging crops and forages throughout Indiana. Not only are ponds and creeks drying up, but pastures are turning brown as well. In many fields, the only green plants are noxious weeds. So, there will be increased chances that livestock will eat toxic plants because there isn’t any grass to eat.

This spring and summer have seen a bumper crop of Poison Hemlock growing along roadsides, in ditches and in some grazing areas. As a consequence, the ADDL has had increased reports of livestock exposed to, and poisoned from eating poison hemlock plants because there was little else to eat other than the poison hemlock. We also have recent reports that White Snakeroot is already growing well, so we can anticipate livestock might resort to eating it as well.

Nitrates - The severe drought has made conditions right for increased uptake of nitrates into cornstalks and other nitrate-accumulating plants. Before using cornstalks, or nitrate-accumulating plants as forage, consider the possibility of toxic levels of nitrate in them and have a representative sample tested for nitrates before using.

The Indiana ADDL at Purdue can test for white snakeroot ingestion in livestock and for the amounts of nitrates in forage.


Blue-Green Algae - the drought and heat have resulted in perfect conditions for blue-green algae growth. Blue-green algae is found in freshwater lakes, streams and ponds where water is warm and stagnant. Blue-green algae is poisonous. While some types of algae are harmless, the blue-green type can produce a natural toxin. Some form toxins that affect the nervous system and others produce toxins that affect the liver. Livestock, pets and wild animals can be poisoned by the toxins produced by some algal blooms. Dogs are particularly susceptible to blue-green algae poisoning because the scum can attach to their coats and be swallowed during self-cleaning.

For videos on these topics please click here

The Purdue Ag Extension Disaster Education (EDEN) website on drought information:
https://ag.purdue.edu/extension/eden/Pages/drought.aspx

The Indiana Board of Animal Health has a blue-green algae website:
http://www.in.gov/boah/2617.htm

A link to the Purdue Agronomy and Extension publication on nitrates by Jim Camberato and Keith Johnson can be found at:
http://www.agry.purdue.edu/ext/forages/Forage%20Analysis%20for%20Nitrate%202012.pdf

A link to the USDA document listing nitrate-accumulating plants can be found at:
http://www.ars.usda.gov/Services/docs.htm?docid=9956

A link to Indiana Plants Poisonous to Livestock and Pets:
http://vet.vet.purdue.edu/toxic/cover1.htm

The Indiana ADDL at Purdue University:
https://www.addl.purdue.edu/
6/28/2012 - Canine Brucellosis Unavailability
Canine Brucellosis Unavailability

Canine brucellosis testing is not being offered at this time due to lack of availability of test kits. We apologize for any inconveniences this may cause.
4/4/2012 - Winter 2012 Indiana Wildlife Disease News
The Indina Wildlife Disease News is a joint project between Indiana DNR, Division of Fish and Wildlife and USDA APHIS Wildlife Services National Wildlife Disease Surveillance and Emergency Response Program.

The purpose of the Indiana Wildlife Disease News is to provide a source of current information for and to facilitate communication among decision makers, stakeholders, and agencies involved with the management of wildlife diseases. The Newsletter will be published quarterly by USDA Wildlife Services and the Indiana Department of Natural Resources. Each quarter, the Newsletter will focus on a disease or wildlife species of current or nearfuture interest.

The current issue can be viewed here (.pdf): Indiana Wildlife Disease News Winter 2012

12/22/2011 - Equine Piroplasmosis Testing Offered
Equine Piroplasmosis testing offered

The Indiana Animal Disease Diagnostic Laboratory is approved to perform equine piroplasmosis analyses for inter- and intrastate movement and will offer testing for Equine Piroplasmosis beginning January 1, 2012.

To be eligible for a waiver of the test fee for state mandated testing, serum samples submitted for Equine Piroplasmosis must state on the ADDL Accession form that the samples are from horses currently belonging to Indiana owners that will be at the Indiana race tracks: Hoosier Park, Anderson, IN, and Indiana Downs, Shelbyville, IN. Samples submitted to ADDL for testing must be drawn by a licensed and accredited veterinarian, the animal ID must be listed, and reason for testing clearly stated on the ADDL submission form.

Testing for international export or for cases with clinical signs compatible with Equine Piroplasmosis must be tested at NVSL.

The charge for non-state mandated piroplasmosis testing for both B. caballi and T. equi will be $24 ($7.00 each for B.caballi and T. equi, plus the $10 ADDL accession fee).
12/13/2011 - Fall 2011 Indiana Wildlife Disease News
The Indina Wildlife Disease News is a joint project between Indiana DNR, Division of Fish and Wildlife and USDA APHIS Wildlife Services National Wildlife Disease Surveillance and Emergency Response Program.

The purpose of the Indiana Wildlife Disease News is to provide a source of current information for and to facilitate communication among decision makers, stakeholders, and agencies involved with the management of wildlife diseases. The Newsletter will be published quarterly by USDA Wildlife Services and the Indiana Department of Natural Resources. Each quarter, the Newsletter will focus on a disease or wildlife species of current or nearfuture interest.

The current issue can be viewed at: http://digitalcommons.unl.edu/icwdmindiana/

or http://extension.entm.purdue.edu/newsletters/wildlife.php
12/6/2010 - New ELISA Prices for Large Submissions
The following ELISA tests are being offered at a discounted rate when submitting 45 or more samples:
Test Type Disease Normal Price (per sample) Discounted Price (per sample)
cELISA Anaplasmosis $8.50 $5.00
cELISA Bluetongue $6.00 $5.50
ELISA Bovine Leukosis $7.00 $5.50
cELISA Caprine Arthritis Encephalitis (CAE) $8.00 $4.50
ELISA Neospora caninum $10.00 $5.00
ELISA Porcine Reproductive and Respiratory Syndrome (PRRS) $5.00 $4.50
For complete test information, see our Tests & Fees page or contact us at addl@purdue.edu
8/25/2010 - University Holiday Schedule
Purdue ADDL and Heeke ADDL will be closed on the following University holidays:
2010
July 5
-
University Observance of Independence Day
September 6
-
Labor Day
November 25-26
-
Thanksgiving Holiday
December 23-24
-
Christmas Holiday
December 30
-
President's Designated Holiday
December 31
-
University Observance of New Year's Day
2011
January 17
-
Martin Luther King, Jr. Day
May 30
-
Memorial Day
July 4
-
Independence Day
September 5
-
Labor Day
November 24-25
-
Thanksgiving Holiday
December 23 & 26
-
Christmas Holiday
December 30
-
President's Designated Holiday
2012
January 2
-
University Observance of New Year's Day
January 16
-
Martin Luther King, Jr. Day
May 28
-
Memorial Day
July 4
-
Independence Day
September 3
-
Labor Day
November 22-23
-
Thanksgiving Holiday
December 24-25
-
Christmas Holiday
December 31
-
President's Designated Holiday
2013
January 1
-
New Year's Day
January 21
-
Martin Luther King, Jr. Day
May 27
-
Memorial Day
7/28/2010 - Externships
Externship Information
The Department of Comparative Pathobiology and the Animal Disease Diagnostic Laboratory (ADDL) offer the opportunity to preview anatomic pathology residency training at an AAVLD-accredited diagnostic laboratory in a veterinary school setting. Externs interact with pathologists, residents and veterinary students in a necropsy service with ample and varied caseload, then follow cases to final diagnosis through correlation of histologic examination and ancillary test results. Histopathology training can be supplemented with biopsy case material. (Download Application/Required Documentation)
In addition to the veterinary library, a resource room in the ADDL provides access to microscopes, internet, PowerPoint presentations by pathology faculty, glass slide study sets complete with case histories, gross pathology images, journals and textbooks. During the academic year, externs can participate in weekly reviews of gross photography from necropsy cases, histopathology seminars, and courses in general or systemic pathology prepared for pathology residents.
Externs also have the opportunity to develop a case report or newsletter article, participate in a research project, or rotate through the branch laboratory (Heeke ADDL) at the Southern Indiana Purdue Agricultural Center, where the caseload is also diverse with many avian accessions.

View Presentation on Careers in Pathology

 

 

 

Contact:
Margaret A. Miller
765-494-7456
pegmiller@purdue.edu